Single molecule analysis of gene expression dynamics

Our Focus
The Tanenbaum group uses cutting-edge live-cell single-molecule microscopy and new types of genetic engineering to uncover the molecular mechanisms of gene expression control in individual cells. Our work focuses both on regulation of human genes in health and disease and on gene regulation of RNA viruses. Using our single-molecule approaches, we aim to uncover how the dynamics and heterogeneity in gene expression affects cell fate. In addition, we are developing new imaging technologies to visualize gene expression dynamics with ever increasing resolution to achieve a deep molecular understanding of these processes.
About Marvin Tanenbaum

Marvin Tanenbaum
My Research
Marvin Tanenbaum received his PhD (Cum Laude) in 2010 from Utrecht University for his work on cell division in the group of Prof. René Medema. During his PhD work, Marvin used live-cell microscopy to study the molecular mechanism of chromosome segregation during cell division. After obtaining his PhD, he received KWF and EMBO fellowships to perform his postdoctoral research in the group of Prof. Ron Vale at UCSF in the United States. As a postdoc, Marvin developed a keen interest in studying the control mechanisms and dynamics of gene expression in single cells, focusing on gene expression control during the cell cycle. He pioneered several new techniques using protein engineering that enabled direct observation of single protein molecules in action, and developed methods to observe gene expression in single living cells by fluorescence microscopy. He also developed a technique to modulate the activity of individual genes in single cells. In 2015, he became a group leader at the Hubrecht Institute. His group studies the molecular mechanisms of gene expression control at the single-molecule level, both for humans and in viruses, and aims to understand how control of gene expression affects important cellular decisions.
Awards
Elected EMBO member in 2022
Recipient of an ERC Consolidator grant in 2022
Prix Galien Excellence COVID-19 Award in 2021
EMBO young investigator (YIP) in 2019
Recipient of a VIDI grant in 2018
Howard Hughes Medical Institute (HHMI) International Research Scholar in 2017
Recipient of an ERC Starting grant in 2015
Recipient of EMBO Long-term fellowship in 2011
Recipient of a Dutch Cancer Society postdoctoral fellowship in 2011
NVMO Oncology Thesis Award in 2011
Utrecht University Thesis Prize in 2011
Key Publications
Boersma S, Rabouw HH, Bruurs LJM, [...] van Kuppeveld F, Tanenbaum ME (2020). Translation and Replication Dynamics of Single RNA Viruses. Cell, 183, 1–16.
Hoek TA, Khuperkar D, Lindeboom RGH, [...] Tanenbaum ME (2019). Single-molecule imaging uncovers rules governing nonsense-mediated mRNA decay. Molecular Cell, 75(2), 324-339.
Boersma S, Khuperkar D, Verhagen BMP, [...] Tanenbaum ME (2019). Multi-color single-molecule imaging uncovers extensive heterogeneity in mRNA decoding. Cell, 178(2), 458-472.
Yan, X., Hoek, T. A., Vale, R. D., & Tanenbaum, M. E. (2016). Dynamics of translation of single mRNA molecules in vivo. Cell, 165(4), 976-989.
Ruijtenberg S, Sonneveld S, Cui TJ, [...] Tanenbaum ME (2020). mRNA structural dynamics shape Argonaute-target interactions. Nat Struct Mol Biol, 28(6), 533.
Krijger PHL, Hoek TA, [...] de Laat W, Tanenbaum ME (2021). A public-private partnership model for COVID-19 diagnostics. Nat Biotechnol. 39(10), 1182-1184.
Members
Marvin Tanenbaum Groupleader | Bernhard Kramer PostDoc | Bram Verhagen PhD Student |
Dhanushika Ratnayake Post Doc | Huib Rabouw Postdoc fellow | Jakob Püschel Phd Student |
Janin Schokolowski Phd student | Leonie Apperloo Technician | Marloes van Drimmelen PhD student |
Matthijs Baars Data scientist | Maximilian Madern Phd Student | Micha Müller PhD Student |
Sora Yang Post Doc | Thomas Sabate Postdoc |